Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells

Figure 1 Cytotoxic effect of Chaga extract on human hepatoma cells. A: The various cells, HepG2, Hep3B, and Chang normal liver cells, were treated with various doses of Chaga extract for 48 h. Cell viability was measured by MTT assay. Data represented as means ± SD of three independent experiments. ^aP < 0.05, ^bP < 0.01, vs control cells; B: The morphological changes of HepG2 cells after exposure to Chaga extract (750 &mgr;g/mL) for 48 h. The photomicrography was taken under a phase contrast microscope (× 200).