Bcl-xL and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells

Figure 1 Mcl-1 and Bcl-x_L expression in CRC. A: The CRC cell lines, HT29, SW620, SW480, and Caco-2, were analyzed for the basal expression of the Bcl-2 family members Bcl-x_L and Mcl-1. Whole cell lysates were prepared, separated, and immunoblotted with antibodies against Bcl-x_L, Mcl-1 and α-tubulin; B: CRC tissues and normal colorectal tissues were tested for mRNA expression (n = 9 patients). mRNA expression levels of Bcl-x_L, Mcl-1 and RPII were measured in all tissue samples by quantitative real-time PCR. mRNA expression levels of Bcl-x_L or Mcl-1 were normalized to RPIIin each sample. Each PCR reaction was run in triplicates. Median is added; C: Immunohistochemical analysis of human CRC tissues was performed as described in the Methods section. All sections included carcinoma as well as normal epithelial tissues to directly compare Bcl-x_L as well as Mcl-1 expression in neoplastic and non-malignant tissues. Representative analysis of immunoperoxidase detection of Bcl-x_L and Mcl-1 in paraffin embedded carcinoma tissue (T) and adjacent non-tumor tissue (N) is presented.