Killing of p53-deficient hepatoma cells by parvovirus H-1 and chemotherapeutics requires promyelocytic leukemia protein

Figure 4 H-1 PV-induced apoptosis is mediated by PML. HepG2 (p53⁺) and HepG2 303 (p53^-) cells were transfected with pSUPER or pSUPER-PML as indicated, and infected with H-1 PV for 48 h. Cells were harvested and subjected to Western blot (A) or PCR analysis (B). Hepatoma cells were treated as described, harvested, and subjected to cytotoxicity assay. Apoptosis was determined as described in Material and Methods (C).