Colorectal Cancer
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. May 28, 2005; 11(20): 3040-3045
Published online May 28, 2005. doi: 10.3748/wjg.v11.i20.3040
Figure 2
Figure 2 Treatment of COLO 205 cells with denbinobin (10-20 μmol/L) for 24 h induced apoptosis. A: FACS analysis of DNA content after 24-h incubation in culture medium supplemented with 10% FCS and 0.1% DMSO or 0-20 μmol/L denbinobin in 0.1% DMSO. Percentages of cells in sub-G1, G0/G1, S, and G2/M phases of the cell cycle determined using established CellFIT DNA analysis software were shown. Increased sub-G1 percentage of COLO 205 cells was shown after treatment with 10-20 μmol/L denbinobin. Two independent experiments were performed in triplicate. aP<0.05 vs DMSO control. B: Electrophoresis of genomic DNA from COLO 205 treated with denbinobin. A typical DNA ladder pattern associated with apoptosis was seen at 10-20 μmol/L denbinobin.