Apoptosis-inducing effect of recombinant Caspase-3 expressed by constructed eukaryotic vector on gastric cancer cell line SGC7901

Figure 1 Schematic map of constructions of recombinant Caspases-3 and eukaryotic expression plasmid pcDNA/Rev-Caspases-3.

Figure 2 Identification of plasmid pBS/Rev-Caspase-3 by PCR with SS-forward and LS-reverse as primers. M: 100 bp ladder DNA marker, 1: PCR product, 882 bp.

Figure 3 Enzyme digestion analysis of plasmid pcDNA/Rev-Caspase-3. M: 100 bp ladder DNA marker, 1: pcDNA/Rev-Caspase-3 cut with Stu I, 2: pcDNA/Rev-Caspase-3 cut with

Figure 4 Comparison of cell count between SGC7901 cells trans-fected with pcDNA3. 1 (+) and pcDNA/Rev. Caspase.

Figure 5 Comparison of MTT assays between SGC7901 cells transfected with pcDNA3. 1 (+) and pcDNA/Rev.Caspase-3.

Figure 6 Electron microscopy of SGC7901 cells transfected with pcDNA3. 1 (+) or pcDNA/Rev.Caspases-3. A: SGC7901 trans-fected with pcDNA/Rev. Caspases-3. Notice the chromatin condensation, crescent formation, original margination (× 5000); B: SGC7901 transfected with pcDNA3.1 (+), Notice no characteristics of apoptosis (× 5000).