Establishment of transgenic mice carrying the gene of human nuclear receptor NR5A2 (hB1F)

Figure 1 PCR results of transgenic mice. 1: DNA molecular weight marker, up 100 bp ladder, down 1 kb ladder; 2: Positive control (pcDNA3-hB1F); 3: Negative control (normal mouse); 4: Water as template; 515: Transgenic founder mice; Up: P1-P2 primers amplified; Down: P3-P4 primers amplified.

Figure 2 Genonic DNA Southern blotting results of transgenic mice. 1: Positive control (pcDNA3-hB1F); 2: Negative control (normal mouse); 313: Transgenic founder mice.

Figure 3 RT-PCR results of transgene expression. 1: DNA molecular weight marker DL 2000; 2: Positive control (pcDNA3-hB1F); 3: Negative control (non-reversely transcribed RNA); 4: C57 mouse liver; 511: Transgenic mice tissues.^aP < 0.05.

Figure 4 Western blotting analysis of transgene expression.

Figure 5 Partial PCR results of F1 transgenic mice. 1: DNA molecular weight marker, up DL 2000, down 100 bp ladder; 2: Positive control (pcDNA3-hB1F); 3: Negative control (normal mouse); 4: Water as control; 512: F1 mice; Up: P1-P2 primers amplified; Down: P5-P6 primers amplified.