Relationship between genetic polymorphism of cytochrome P450IIE1 and fatty liver

Figure 1 Electrophoregram of PCR products (Lanes 1-5). The signals of pUC19-DNA marker (M₁) present 489 bp, 404 bp, 331 bp, 242 bp, 190 bp, 147 bp and 110 bp from up to bottom. The signals of DL2000-DNA marker (M₂) present 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp and 100 bp from up to bottom.

Figure 2 After digested with restriction enzyme PstI (A) or RsaI (B), CYPIIE1 was divided into three types, namely wild type homozygote (C1/C1) (Lane 1), heterozygote (C1/C2) (Lane 2) and mutant homozygote (C2/C2) (Lane 3). C1 means wild type gene (PstI+, RsaI-), and C2 mutant gene (PstI-, RsaI+). pUC19-DNA marker (M1) and DL2000-DNA marker (M₂).