The effect pathway of retinoic acid through regulation of retinoic acid receptor a in gastric cancer cells

Figure 1 Expressions of RARα, RARβ and RXRα in gastric cancer cell lines detected by Northern blot. Cells were treated with 10^-6 mol•L¯¹ ATRA.

Figure 2 A: Expression of RARα mRNA in BGC-823 cells transfected with antisense RARα gene; B: Expression of RARα mRNA in MKN-45 cells transfected with sense RARα gene.

Figure 3 A: Growth inhibitory effect of ATRA on gastric cancer cell lines measured by the method of MTT, Cells were treated with various concentrations of ATRA indicated; B: Growth inhibitory effect of ATRA on BGC-823 cells transfected with antisense RARα gene and on MKN-45 cells transfected with sense RARα gene, respectively.

Figure 4 A: Regulation of ATRA on βRARE transcriptional activity in gastric cancer cell lines detected by CAT assay; B: Regulation of ATRA on βRARE transcriptional activity in BGC-823 cells transfected with antisense RARα gene and in MKN-45 cells transfected with sense RARα gene, respectively.

Figure 5 A: Inhibitory effect of ATRA on AP-1 activity in gastric cancer cell lines at various concentrations of ATRA shown by CAT assay; B: Effect of ATRA on AP-1 activity in BGC-823 cells transfected with antisense RARα gene and in MKN-45 cells transfected with sense RARα gene, respectively.