Apoptosis induced by norcantharidin in human tumor cells

Figure 1 Control BEL-7402 cells, W-G staining. × 350

Figure 2 BEL-7402 cells treated with 10 mg/L NCTD for 24 h. (↑) indicates multipolar distribution of chromosomes in postmitotic cells. (▲) nuclear fragments in apoptotic cells, (-) indicates con densed cells, (AB) apoptotic bodes containing one or two nuclear fragments. W-G staining, × 350

Figure 3 Ultrastructural features of BEL-7402 cells treated with 10 mg/L NCTD for 24 h. Endoplasmic reticulum (ER) dilated in the apoptotic cell (↑), the apoptotic bodies characterized by compaction of nuclear chromatin and condensation of cytoplasm. × 14000

Figure 4 Agarose gel electrophoresis of DNA extracted from BEL-7402 cells. M: DNA marker; 1: control group; 2: group treated with 10 mg/L NCTD for 24 h.

Figure 5 Western blotting analysis of Bcl-2 protein in BEL-7402 cells. 1: control; 2: treated with 10 mg/L NCTD for 24 h; 3: treated with 10 mg/L NCTD for 48 h; 4: treated with 10 mg/L NCTD for 72 h.