Expression of Notch pathway components (Numb, Itch, and Siah-1) in colorectal tumors: A clinicopathological study

Figure 1 Notch (A1, B1 and C1), Numb (A2, B2 and C2), Itch (A3, B3 and C3), and Siah-1(A4, B4 and C4) immunohistochemical expression in the three groups. Expression of Notch is higher in CRCs (C1-4), and that of Numb Itch and Siah-1 is higher in adenomas (B1-4) and controls (A1-4).

Figure 2 Survival of patients according to Notch (A), Numb (B), Itch (C), and Siah-1 (D) immunohistochemical expression, lymph node metastasis (E), and stage (F). Survival of patients with Notch expression is shorter than that of those without Notch expression. In contrast, expression of Numb, Itch, and Siah-1 is related to better survival.

Figure 3 Relative gene expression levels of Notch, Numb, Itch, and Siah-1 in 50 patients diagnosed with colorectal carcinoma (qPCR analysis).

Figure 4 Levels of Notch, Numb, Itch, and Siah-1 expression in adenomas and carcinomas. Expression levels of Notch are higher in colorectal carcinoma; in contrast, expression levels of Numb, Itch, and Siah-1 are higher in adenomas (qPCR analysis).

Figure 5 Distribution of relative gene expression levels of Notch, Numb, Itch, and Siah-1 according to grade (A), lymph node metastasis (B), stage (C) and survival (D) in colorectal carcinoma (qPCR analysis).

Figure 6 Survival of patients according to expression levels of Notch (A), Numb (B), Itch (C), and Siah-1 (D). Survival of patients with Notch upregulation is shorter than that of patients without Notch upregulation. Conversely, upregulation of Numb, Itch, and Siah-1 is related to better survival.