Update on a tumor-associated NADH oxidase in gastric cancer cell growth

Figure 1 Tumor-associated NADH oxidase protein expressions in different human gastric cancer lines. Aliquots of cell lysates were separated by SDS-PAGE and analyzed for tNOX expression by western blot analysis. β-Actin was used as an internal control to monitor for equal loading. Gastric cancer cell lines including, AGS (gastric adenocarcinoma), TMC-1 (derived from the lymph node of a moderately differentiated stomach adenocarcinoma), MKN45 (from the lymph node of a moderately differentiated stomach adenocarcinoma), SNU-1 (from a poorly differentiated primary carcinoma of the stomach), TMK-1 (from a poorly differentiated adenocarcinoma), and SCM (human gastric carcinoma), were used to study the expression of tNOX protein. SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis; tNOX: Tumor-associated NADH oxidase.

Figure 2 Cell growth monitored by cell impedance technology. For continuous monitoring of dynamic changes in cell proliferation, cells (10⁴ cells/well) were seeded onto E-plates and incubated for 30 min at room temperature, after which E-plates were positioned onto the Real-Time Cell Analysis station (Roche, Mannhein, Germany). Cells were grown, and impedance was determined every hour, as previously described[21,22]. Cell impedance is defined by the cell index (CI) = (Z_i - Z₀) [Ohm]/15[Ohm], where Z₀ is background resistance and Z_i is the resistance at an individual time point. A normalized cell index was determined as the cell index at a certain time point (CI_ti) divided by the cell index at the normalization time point (CI_{nml_time}). Normalized cell index values measured over 92 h are shown. Gastric cancer cell lines, including AGS (gastric adenocarcinoma), TMC-1 (derived from the lymph node of a moderately differentiated stomach adenocarcinoma), MKN45 (from the lymph node of a moderately differentiated stomach adenocarcinoma), and SCM (human gastric carcinoma), were used to study cell growth pattern using cell impedance measurements.