Inhibitory effect of TongXie-YaoFang formula on colonic contraction in rats

Figure 1 Effects of TongXie-YaoFang formula on movement index and contraction frequency of colonic longitudinal smooth muscle strips in rats. A: TongXie-YaoFang (TXYF) formula significantly reduced the movement index (MI) of colonic longitudinal smooth muscle strips (CLSMs) in neonatal maternal separation plus restraint stress (NMS + RS) rats; B: TXYF formula significantly reduced contraction frequency (CF) of CLSMs in NMS + RS rats; C: CF in the three groups after treatment. ^bP < 0.01 vs the normal group; ^dP < 0.01 vs the NMS + RS group.

Figure 2 Effects of TMB-8,4-aminopyridine and nifedipine on movement index of colonic longitudinal smooth muscle strips in rats. A: After treatment with 8-(N,N-diethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride (TMB-8), 4-aminopyridine (4-AP) and nifedipine, the movement index (MI) of colonic longitudinal smooth muscle strips (CLSMs) in the TongXie-YaoFang (TXYF) formula group was lower than that in the normal group (n = 8, P < 0.05); B: After treatment with TMB-8, MI of rat CLSMs in the TXYF formula group was lower than that in the normal group. ^aP < 0.05 vs normal group.

Figure 3 Effects of apamin, nifedipine, apamin, N-ethylmaleimide, methylene blue, methylene blue and N^G-nitro-L-arginine methyl ester on movement index of colonic longitudinal smooth muscle strips in rats. NEM: Nifedipine, apamin, N-ethylmaleimide, Methylene blue; L-NAME: N^G-nitro-L-arginine methyl ester; MI: Movement index; CLSMs:Colonic longitudinal smooth muscle strips.