Original Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 7, 2013; 19(9): 1405-1415
Published online Mar 7, 2013. doi: 10.3748/wjg.v19.i9.1405
Figure 1
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Figure 1 Representative images of schistosomal hepatic fibrosis in the groups over time. Collagen fibers are stained blue and arrows show egg granulomas. Original magnification 100×. Histogram shows the percentage of collagen which represented the degree of hepatic fibrosis. bP < 0.01 vs group A; dP < 0.01 vs group B; fP < 0.01 vs group C; hP < 0.01 vs week 9.
Figure 2
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Figure 2 Representative images of immunostaining for transforming growth factor-beta 1 and alpha-smooth muscle actin in the groups over time. Target protein positive staining is yellow, brownish-yellow or snuff. Original magnification 100×. Histogram shows integral optical densities of target proteins. TGF-β1: Transforming growth factor-beta 1; α-SMA: Alpha-smooth muscle actin; IOD: Integral optical density. bP < 0.01 vs group A; dP < 0.01 vs group B; fP < 0.01 vs group C; hP < 0.01 vs week 9.
Figure 3
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Figure 3 Representative images of immunostaining for pSmad2/3 and Smad7 in the groups over time. Target protein positive staining is yellow or brownish-yellow. Original magnification 100×. Histogram shows integral optical densities of target proteins. bP < 0.01 vs group A; dP < 0.01 vs group B; fP < 0.01 vs group C; hP < 0.01 vs week 9. IOD: Integral optical density.
Figure 4
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Figure 4 Representative electrophoretic bands of transforming growth factor-beta 1, alpha-smooth muscle actin, pSmad2, pSmad3 and Smad7 mRNA (reverse transcription-polymerase chain reaction). Numbers 1-3 represent group A, B and C, respectively, at 9 wk; Numbers 4-6 represent group A, B and C, respectively, at 15 wk. The 132 bp glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA fragment was used as an internal control. M: Marker; TGF-β1: Transforming growth factor-beta 1; α-SMA: Alpha-smooth muscle actin.
Figure 5
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Figure 5 Representative electrophoretic bands of transforming growth factor-beta 1, alpha-smooth muscle actin, pSmad2/3 and Smad7 protein (Western blotting). Numbers 1-3 represent group A, B and C, respectively, at 9 wk. Numbers 4-6 represent group A, B and C, respectively, at 15 wk. The 37 kD glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. TGF-β1: Transforming growth factor-beta 1; α-SMA: Alpha-smooth muscle actin.
Figure 6
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Figure 6 Relative mRNA expressions (target gene integral optical density/glyceraldehyde-3-phosphate dehydrogenase integral optical density, reverse transcription-polymerase chain reaction) of transforming growth factor-beta 1, alpha-smooth muscle actin, pSmad2, pSmad3 and Smad7. TGF-β1: Transforming growth factor-beta 1; α-SMA: Alpha-smooth muscle actin. bP < 0.01 vs group A; dP < 0.01 vs group B; fP < 0.01 vs group C; hP < 0.01 vs week 9.
Figure 7
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Figure 7 Relative protein expressions (target protein integral optical density/glyceraldehyde-3-phosphate dehydrogenase integral optical density, Western blotting) of transforming growth factor-beta 1, alpha-smooth muscle actin, pSmad2/3 and Smad7. TGF-β1: Transforming growth factor-beta 1; α-SMA: Alpha-smooth muscle actin. bP < 0.01 vs group A; dP < 0.01 vs group B; fP < 0.01 vs group C; hP < 0.01 vs week 9.