Expression of HER2 and bradykinin B1 receptors in precursor lesions of gallbladder carcinoma

Figure 1 Expression of immunoreactive HER2 and bradykinin B₁ receptor in pyloric- and intestinal-type adenomas. Tissue sections were incubated with each antibody and then the biotin/streptavidin-peroxidase technique was followed. B₁R: B₁ receptor.

Figure 2 Immunoreactive HER2 and bradykinin B₁ receptor receptors in normal gallbladder, invasive carcinoma and in epithelia with intestinal metaplasia and carcinoma in situ. Biotin/streptavidin-peroxidase technique. B₁R: B₁ receptor; GC: Goblet cell.

Figure 3 High magnification of gallbladder metaplastic epithelium showing immunoreactivity for HER2 (A) and bradykinin B₁ receptor receptor (B). Arrows show the limit between apical and basolateral cell membrane domains. C-G: Fluorescence in situ hybridization for HER2. Tissue sections were hybridized with a mixture of HER2-Texas Red and cen-17 labeled with fluorescein isothiocyanate. C: Normal gallbladder epithelium; D: Epithelium with intestinal metaplasia; E: Carcinoma in situ; F: Invasive carcinoma; G: Positive control corresponding to a breast cancer sample classified as HER2 + 3.