An end-to-end anastomosis model of guinea pig bile duct: A 6-mo observation

Figure 1 Microsurgical reconstruction of common bile duct with end-to-end anastomosis.

Figure 2 Gross observation of normal common bile duct (A) and its diameter (B) 2 mo after end-to-end anastomosis. Asterisk indicates anastomosed region in 2-mo group.

Figure 3 Histological examination of bile duct in all groups (original magnification, × 200). A: Normal bile duct intima; B: Gland proliferation and infiltration of inflammatory cells in 2-mo group; C: Fibrous thickening of bile duct and dense infiltration of inflammatory cells in 3-mo group; D: Epithelial proliferation as well as intra- and extramural glandular hyperplasia in bile duct.

Figure 4 Proliferating cell nuclear antigen immunohistochemical staining. A: Distribution of proliferating cell nuclear antigen (PCNA) positive cells in normal bile duct (original magnification, × 100); B, C: Positive PCNA cells distributed in glandular-epithelia and peribiliary glands in 2- and 3-mo groups, respectively (B: original magnification, × 100; C: original magnification, × 200); D: Positive PCNA cells located mainly in glandular elements and epithelium cells (original magnification, × 100).