Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells

Figure 1 Effect of stromal cell-derived factor-1 (20 ng/mL) on E-cadherin expression (× 400). A: E-cadherin expression in HT29 cells; B: Significantly lower E-cadherin expression level 48 h after incubated with stromal cell-derived factor-1 (SDF-1) (P < 0.05); C: AMD3100- inhibited E-cadherin expression; D: No effect of AMD3100 alone on E-cadherin expression. Arrows mean the expression of E-cadherin; Bars indicate mean ± SD of six random fields. ^aP < 0.05.

Figure 2 Effect of stromal cell-derived factor-1 (20 ng/mL) on E-cadherin mRNA expression after 24 h (A) and 48 h (B) in different groups. Bars represent mean ± SD of three individual experiments. ^aP < 0.05. SDF-1: Stromal cell-derived factor-1.

Figure 3 Effect of stromal cell-derived factor-1 (20 ng/mL) on β-catenin expression after 48 h (× 400). A: β-catenin expression in HT29 cells; B: Significantly lower β-catenin expression level 48 h after incubated with stromal cell-derived factor-1 (SDF-1) (P = 0.031); C: AMD310-inhibited β-catenin expression; D: No effect of AMD3100 alone on β-catenin expression. Arrows mean the expression of β-catenin; Bars indicate mean ± SD of six random fields. ^aP < 0.05.

Figure 4 Effect of stromal cell-derived factor-1 (20 ng/mL) on β-catenin mRNA expression after 24 h (A) and 48 h (B) in different groups. Bars represent mean ± SD of three individual experiments. ^aP < 0.05. SDF-1: Stromal cell-derived factor-1.

Figure 5 Effect of stromal cell-derived factor-1 on the phosphorylation of PI3K/AKT and β-catenin. A: Phosphorylation of PI3K/AKT and β-catenin after incubated with stromal cell-derived factor (SDF) at different periods of time (1, 5, 10, 15, 30, 60, 120 min and on days 1 and 2); B: Phosphorylation of PI3K/AKT and β-catenin after incubated with SDF-1 at different concentrations (5, 10, 20, 40 and 100 ng/mL) for 30 min; C and D: SDF-1 increases phosphorylation of PI3K/AKT and β-catenin in HT29 cells in a time- and dose-dependent manner. Bars represent mean ± SD of triplicate experiments. ^aP < 0.05.

Figure 6 MD3100 and LY294002 inhibit stromal cell-derived factor-1-induced phosphorylation of PI3K/AKT and β-catenin while AMD3100 or LY294002 alone has no effect on phosphorylation of PI3K/AKT and β-catenin. SDF-1: Stromal cell-derived factor-1.