Original Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Jul 14, 2011; 17(26): 3109-3116
Published online Jul 14, 2011. doi: 10.3748/wjg.v17.i26.3109
Figure 1
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Figure 1 CCL20 mRNA expression relative to PMM1 and β2M in colorectal liver metastasis patients with (n = 53) and without (n = 29) FOLFOX chemotherapy before colorectal liver metastasis surgery (A) and itemized according to different time periods of FOLFOX treatment before colorectal liver metastasis surgery (no FOLFOX therapy, n = 29; FOLFOX treatment ≤ 1 mo, n = 14; FOLFOX treatment ≤ 1 year, n = 22; FOLFOX treatment > 1 year, n = 17) (B). Q-RT-PCR data are expressed as mean ± SE, aP < 0.05 and bP < 0.001, respectively. CRLM: Colorectal liver metastasis.
Figure 2
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Figure 2 CCL20 protein concentrations (pg/mL pro mg total protein) in colorectal liver metastasis patients with (n = 53) and without (n = 29) FOLFOX chemotherapy before colorectal liver metastasis surgery (A) and itemized according to different time points of FOLFOX treatment before colorectal liver metastasis surgery (no FOLFOX therapy, n = 29; FOLFOX treatment ≤ 1 mo, n = 14; FOLFOX treatment ≤ 1 year, n = 22; FOLFOX treatment > 1 year, n = 17) (B). Protein data are expressed as mean ± SE, aP < 0.05 and bP < 0.001, respectively. CRLM: Colorectal liver metastasis.
Figure 3
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Figure 3 Immunohistochemical staining (original magnification × 200). Representative examples of CCL20 (A, B), CCR6 (C, D) and Ki-67 (E, F) protein expression in colorectal liver metastasis specimens from a colorectal liver metastasis patient without (A, C, E) and with preoperative FOLFOX treatment less than 1 mo before surgery (B, D, F), respectively.
Figure 4
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Figure 4 CCR6 mRNA expression relative to PMM1 and β2M in CRLM patients with (n = 53) and without (n = 29) FOLFOX chemotherapy before CRLM surgery (A) and itemized according to different time points of FOLFOX treatment before CRLM surgery (no FOLFOX therapy, n = 29; FOLFOX treatment ≤ 1 mo, n = 14; FOLFOX treatment ≤ 1 year, n = 22; FOLFOX treatment > 1 year, n = 17) (B). Q-RT-PCR data are expressed as mean ± SE, aP < 0.05.
Figure 5
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Figure 5 Expression of chemokine receptor CCR6 in colorectal liver metastasis patients itemized according to different time points of FOLFOX treatment before colorectal liver metastasis surgery as determined by Western blotting analysis. Total cell lysates of tumor (P) were immunoblotted with antibodies specifically recognizing chemokine receptor CCR6. Acute leukemia cell line HL60 served as a positive control for the detection of CCR6.