Long-term effects of lamivudine treatment in Japanese chronic hepatitis B patients

Figure 1 Melting curve analysis using the LightCycler probe hybridization assay. The melting curves were converted to melting peaks by plotting the negative derivative of fluorescence with respect to temperature [-d(F3)/dT]. The melting temperature (Tm) for each of the YMDD mutants is indicated with a vertical line. A: Representative results for differentiating HBV YMDD mutants. YMDD, YVDD and YIDD mutants showed distinct Tm values; B: The shoulder curve indicated the mixed mutant-type. Representative results show YMDD + YIDD mutants.

Figure 2 Change in the proportion of YMDD wild, mixed mutant, and mutant-types during lamivudine treatment.