Protective effects of 2,4-dihydroxybenzophenone against acetaminophen-induced hepatotoxicity in mice

Figure 1 Effects of 2,4-dihydroxybenzophenone administration on serum levels of alaine aminotransferase, aspartate aminotransferase and lactate dehydrogenase in mice. Acetaminophen (APAP) was administered at 350 mg/kg sc and 2,4-dihydroxybenzophenone (BP-1) was administered ig at doses of 200, 400 and 800 mg/kg respectively 4 d before acetaminophen treatment. Alaine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase(LDH) levels were measured 24 h after acetaminophen treatment. Results were expressed as mean ± SE and data was analyzed by one-way analysis of variance using SPSS 13.0 software. ^aP < 0.05 vs acetaminophen group; ^cP < 0.05 vs control group.

Figure 2 Liver histology of all groups 24 h after acetaminophen treatment (350 mg/kg). Hematoxylin-eosin-stained liver sections displayed representative hepatocellular morphological changes. Original magnification × 200. A: Liver section in the vehicle control group showed a normal lobular structure; B: Liver section in acetaminophen alone group showed large areas of centrilobular necrosis, vacuolar degeneration and inflammatory cell infiltration; C: Liver section in the 200 mg/kg group showed necrosis with the same degree as in acetaminophen group; D: Liver section in the 400 mg/kg group showed a significant alleviation of liver injury; E: Liver section in the 800 mg/kg group showed absence of necrosis and almost normal lobular structure.