Association of p53/p21 expression with cigarette smoking and prognosis in esophageal squamous cell carcinoma patients

Figure 1 Immunohistochemical staining for p53 in an esophageal squamous cell carcinoma exhibiting expression. A: With primary antibody, showing reactivity (brown nuclear staining of some tumor cells) (× 100); B: With primary antibody, showing reactivity (× 400); C: With primary antibody, no reactivity (× 100). Sections were counter-stained with hematoxylin.

Figure 2 Immunohistochemical staining for p21 in an esophageal squamous cell carcinoma exhibiting expression. A: With primary antibody, showing reactivity (brown nuclear staining of some tumor cells) (× 100); B: With primary antibody, showing reactivity (× 400); C: With primary antibody, no reactivity (× 100). Sections were counter-stained with hematoxylin.

Figure 3 Kaplan-Meier survival curves for esophageal squamous cell carcinoma patients with or without p21 over-expression. A: Overall survival curves were classified by p21 over-expression in the whole series of ESCC patients; B: Survival curves in the operable group of ESCC patients, stratified according to p21 over-expression; C: Survival curves in the operable group of patients with positive expression of p53, stratified according to p21 over-expression.