Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector

Figure 1 Characterization of polymerase chain reaction (PCR) amplification by electrophoresis. A: Amplification of calreticulin (CRT)/hepatitis B surface antigen (HBsAg) fusion gene by PCR. Lane 1: Negative control; Lane 2: CRT/HBsAg fusion gene recombinant pJW4303 plasmid; Lane M: DNA marker; B: Characterization of CRT/HBsAg fusion gene recombinant pENTR/D-TOPO transfer vector by PCR. Lane 1: Negative control; Lane 2: CRT/HBsAg fusion gene recombinant pENTR/D-TOPO transfer vector amplified by specific PCR; Lane M: DNA marker.

Figure 2 Culture of recombinant adenovirus in HEK293A cells. A: CPE was observed in recombinant adenovirus-containing HEK293A cells; B: Normal HEK293A cells cultured as control.

Figure 3 Characterization of CRT/HBsAg fusion protein expression by Western blotting. The expression of CRT/HBsAg fusion protein in 293A cells was determined in 293A cells transfected with Ad-CRT/HBsAg and Ad-LacZ by Western blotting analysis. Human anti-HBsAg positive serum was used at a 1:100 dilution for the detection of CRT/HBsAg fusion protein expression. Lane 1: Lysate from 293 cells transfected with Ad-LacZ; Lane 2: Lysate from 293 cells transfected with Ad-CRT/HBsAg.