Influence of paeonol on expression of COX-2 and p27 in HT-29 cells

Figure 1 Effect of different concentrations (0-1. 504 mmol/L) of paeonol on proliferation of HT-29 cells. The inhibition of cell proliferation showed a dose- as well as time-dependent increase.

Figure 2 Expression of COX-2 (A) and p27 (B) in HT-29 cells induced by paeonol (× 400).

Figure 3 Flow cytometry detects apoptosis of HT-29 cells induced by paeonol. A: Controls; B: 0.094 mmol/L paeonol; C: 0.376 mmol/L paeonol; D: 1.504 mmol/L paeonol.

Figure 4 Changes in COX-2 and p27 induced by paeonol. After exposure to different concentrations for 24 and 48 h, HT-29 cells were observed to have a dose-dependent decrease in COX-2, but an increase in p27. GAPDH acted as an internal control.