Gastric motor effects of ghrelin and growth hormone releasing peptide 6 in diabetic mice with gastroparesis

Figure 1 Effect of increasing doses of ghrelin (0-200 &mgr;g/kg, ip) or GHRP-6 on gastric emptying in DM mice. Bars and error bars represent the mean ± SE of at least six animals. ^aP < 0.05 vs normal saline (NaCl).

Figure 2 Effects of atropine, L-NAME and D-Lys³-GHRP-6 on the gastroprokinetics of ghrelin or GHRP-6 in the DM mice. Mice were pretreated with atropine (1 mg/kg), L-NAME (50 mg/kg) or D-Lys³-GHRP-6 (5 &mgr;mol/kg) before administration of ghrelin (100 &mgr;g/kg) or GHRP-6 (100 &mgr;g/kg), and the effects were compared with those of treatment with ghrelin or GHRP-6 (100 &mgr;g/kg) alone. Bar graph and error bars represent the means ± SE of at least six animals. ^aP < 0.05 vs treatment with ghrelin or GHRP-6 (100 &mgr;g/kg) alone.

Figure 3 Expression of GHS-R mRNA in gastric fundic strips from DM mice. The band at 217 bp corresponds to the amplified GHS-R cDNA product with the expected length. The band at 260 bp corresponds to the amplified β-actin cDNA product with the expected length.