Isolation and bioinformatics analysis of differentially methylated genomic fragments in human gastric cancer

Figure 1 Schematic diagram of MS-RDA experimental strategy.

Figure 2 Amplicons of gastric cancer and normal gastric mucosa. M: DNA marker; 1: Gastric cancer amplicon; 2: Normal gastric mucosa amplicon.

Figure 3 A: Results after the third cycle of competitive hybridization. B: After a second cycle of competitive hybridization. M: DNA marker; 1: differentially methylated fragment of approximately 400 bp; 2: Two differentially methylated fragments, one is approximately 400 bp and the other lies between 200-300 bp.

Figure 4 PCR results. M: DNA marker; 1: Differ-entially methylated fragment of approximately 400 bp; 2: Differentially methylated fragment of approximately 200-300 bp.

Figure 5 AY887106 (A) and AY887107 (B) sequencing.