Ghrelin and gastric acid secretion

Figure 1 Effects of rat ghrelin (A) and gastrin-17 (B) on acid output in gastric lumen-perfused rats. A: Time course of acid output by IV administration of ghrelin (0.04, 0.8, 4, 20 μg/kg) in gastric lumen perfused rats; B: Time course of acid output by IV administration of gastrin (0.8, 8, 13.2 and 20 μg/kg) in gastric lumen perfused rats. Saline was administered in control rats. Each value represents the mean ± SE of acid output at 15 min intervals. n = 4, 5, 6, 7. Reprinted with permission[30].

Figure 2 Effect of famotidine on ghrelin-stimulated acid output in gastric lumen-perfused rats. Time course of acid output by the IV administration of famotidine (0.33 mg/kg) and ghrelin (20 μg/kg) in gastric lumen-perfused rats. Famotidine was administered intravenously 15 min before ghrelin injection. Each value represents the mean ± SE of acid output at 15 min intervals. n = 4. Reprinted with permission[30].

Figure 3 Effect of ghrelin on the concentration of HDC mRNA in rat gatsric mucosa. Ghrelin (20 μg/kg) was administered intravenously. The concentration of HDC mRNA was measured by real time RT-PCR using LightCycler. Control: The concentration of HDC mRNA in gastric mucosa of rats with saline administration. 1 h; the concentration of HDC mRNA in gastric mucosa of rats with ghrelin administration, the stomachs were excised 1 h after the administration. 2 h; the concentration of HDC mRNA in gastric mucosa of rats with ghrelin administration, the stomachs were excised 2 h after the administration. Each value represents the mean ± SE of HDC mRNA demonstrated as % of control. n = 28, 20, 20. ^bP < 0.01. Reprinted with permission[30].

Figure 4 Effect of vagotomy on the concentration of HDC mRNA in rat gastric mucosa stimulated by ghrelin administration. Subdiaphragmatic vagotomy was performed in rats 3 h or 7 d before the experiments. Ghrelin (20 µg/kg) was administered IV. The concentration of HDC mRNA was measured by real time RT-PCR using Light Cycler. Control: The concentration of HDC mRNA in gastric mucosa of rats with saline administration. Ghrelin: The concentration of HDC mRNA in gastric mucosa of rats with ghrelin administration, the stomachs were excised 2 h after the administration. 3 h after vagotomy, the experiments were performed 3 h after vagotomy. 1 wk after vagotomy, the experiments were performed 7 d after vagotomy. Each value represents the mean ± SE of HDC mRNA demonstrated as % of control. n = 8 or 15. NS: Not significant. Reprinted with permission[30].