Inhibition of pancreatic carcinoma cell growth in vitro by DPC4 gene transfection

doi:10.3748/wjg.14.6254

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Oct 28, 2008 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 28, 2008; 14(40): 6254-6260
Published online Oct 28, 2008. doi: 10.3748/wjg.14.6254

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Figure 1 The mRNA of DPC4 by RT-PCR. A: Non-malignant pancreatic species; B: Malignant pancreatic species.

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Figure 2 Immunohistochemical staining (× 100). A: Intense positive expression of DPC4 in the nuclei and/or cytoplasm in the normal pancreatic tissue; B: Weak expression of DPC4 in normal pancreatic tissue; C: Pancreatic carcinoma showed loss of DPC4 expression; D: DPC4 expression in an adenocarcinoma with a wild-type DPC4 gene; E: Intense positive expression of DPC4 in the malignant pancreatic tissue.

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Figure 3 PCR amplification for the DPC4 gene. A: PCR product; M: Marker.

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Figure 4 The expression of DPC4 mRNA in BxPC-3. M: Marker; A: BxPC-3/-; B: BxPC-3/pLXSN; C: BxPC-3/DPC4.

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Figure 5 Westen blot for DPC4 in BxPC-3 cells. A: BxPC-3/DPC4; B: BxPC-3/pLXSN; C: BxPC-3/-.

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Figure 6 Cell growth curve. P = 0.0209, BxPC-3/DPC4 vs BxPC-3/pLXSN; P = 0.0160, BxPC-3/DPC4 vs BxPC-3/-.

Citation: Shen W, Tao GQ, Li DC, Zhu XG, Bai X, Cai B. Inhibition of pancreatic carcinoma cell growth in vitro by DPC4 gene transfection. World J Gastroenterol 2008; 14(40): 6254-6260
URL: https://www.wjgnet.com/1007-9327/full/v14/i40/6254.htm
DOI: https://dx.doi.org/10.3748/wjg.14.6254