Hypermethylated SFRP2 gene in fecal DNA is a high potential biomarker for colorectal cancer noninvasive screening

Figure 1 Methylation status of SFRP2 gene in preoperative and postoperative fecal specimens and in CRC tissues taken from the same patients. P: Patient; M: Methylated; U: Unmethylated.

Figure 2 Methylation status of SFRP2 gene in tissue and feces taken from the same patients with benign colorectal diseases and normal controls.

Figure 3 Prevalence of hypermethylated SFRP2 in stool and tissue specimens taken from the subjects with normal colonic mucosa, hyperplastic polyps (HP), adenomas and colorectal cancers (CRCs).

Figure 4 The photofluorogram of hypermethylated SFRP2 gene in fecal specimen performed by MethyLight. A: Photofluorogram of SFRP2 gene performed in M (methylation) primer and its amplification condition; B: Photofluorogram of SFRP2 gene performed in U (unmethylation) primer and its amplification condition. P represents the patients; and NTC represents the negative controls.