Inhibitory effects of genistein and resveratrol on guinea pig gallbladder contractility in vitro

Figure 1 Sample traces showing the basal contractile activity of the gallbladder before and after the administration of 20 μmol/L genistein (Gen), resveratrol (Res) and 17β-estradiol (Est).

Figure 2 Effects of genistein (Gen), resveratrol (Res) and 17β-estradiol (Est) on resting tension (A), mean contractile amplitude (B) and (C) mean frequencies of phasic contraction in isolated guinea pig gallbladder muscle strips (n = 10). ^aP < 0.05 vs solvent control.

Figure 3 Effects of genistein (Gen, 10 μmol/L) and resveratrol (Res, 10 μmol/L) on the basal tension (A) and mean amplitude (B) of phasic contraction in isolated guinea pig gallbladder muscle strips after preincubation with ICI 182780 (ICI) or bpV (phen) (bpV) (n = 5). ^aP < 0.05 vs corresponding Gen or Res group.

Figure 4 Traces of ACh and CaCl₂-induced contraction of gallbladder muscle strip in Ca²⁺-free Kreb’s solution in the absence and presence of genistein (Gen, 20 μmol/L).

Figure 5 A: Line plots showing effects of genistein (Gen, 40 μmol/L), resveratrol (Res, 40 μmol/L) and 17β-estradiol (Est, 40 μmol/L) on ACh concentration-dependent contraction curves in isolated guinea pig gallbladder muscle strips (n = 6-7); B: Effects of genistein (Gen, 40 μmol/L), resveratrol (Res, 40 μmol/L) and 17β-estradiol (Est, 40 μmol/L) on KCl concentration-dependent contraction curves in isolated guinea pig gallbladder muscle strips (n = 12).