Effect and mechanism of the Twist gene on invasion and metastasis of gastric carcinoma cells

Figure 1 Western blot analysis of Twist expression in MKN28, pcDNA3 control and TwistS transfectant clones (C1, C2 and C3).

Figure 2 Cell adherence rate, and cell migration and cell invasion ability. A: Adherence rate in three cell lines; B: Migration ability in three cell lines; C: Invasion ability in three cell lines. ^aP < 0.05 vs TwistS-MKN28 cells.

Figure 3 Livers from nude mouse models of gastric cancer cell metastasis inoculated with: (A) MKN28; (B) pcDNA3-MKN28; (C) TwistS-MKN28 cells.

Figure 4 Twist promoted Tcf-4 /Lef DNA binding activity with EMSA. Lane 1: Free probe; Lane 2: Competitor; Lane 3:MKN28 cells; Lane 4:pcDNA3 control cells; Lanes 5-7: Three TwistS clones.

Figure 5 Twist increased expression of cyclin D1 mRNA by RT-PCR. Lane 1:Marker; Lane 2: MKN28 cells; Lane 3: pcDNA3 control cells; Lanes 4-6: Three TwistS clones.

Figure 6 Western blot analysis in each cell line. A: Expression of MMP-2 in MKN28 cells, pcDNA3 control cells and TwistAS cells by Western blot analysis; B: Western blot analysis of Twist expression in MKN45, pcDNA3 control and TwistS transfectants clone (C1, C2 and C3).

Figure 7 Down-regulation of Twist-inhibited invasion in MKN45 cells. Representative photographs of invasion in three cell lines (hematoxylin-eosin staining, × 200 magnification). A: MKN45; B: pcDNA3-MKN45; C: TwistAS-MKN45.

Figure 8 Suppression of Twist-inhibited expression of cyclin D1 mRNA by RT-PCR. Lane 1: Marker; Lane 2: MKN45 cells; Lane 3: pcDNA3 control cells; Lanes 4-6: Three TwistAS clones.

Figure 9 Comparison of activity of MMP-2 by gelatin zymography assay.