Basic Research
Copyright ©2008 The WJG Press and Baishideng.
World J Gastroenterol. Apr 7, 2008; 14(13): 2023-2028
Published online Apr 7, 2008. doi: 10.3748/wjg.14.2023
Figure 1
Figure 1 Concentration of GAL-3 in SPSC and SSW (ELISA).
Figure 2
Figure 2 GAL-3 mRNA of SW1990 cells detected by RT-PCR. M: Marker; 1: GAPDH of control group; 2: GAPDH after stimulation with 40% SPSC; 3: GAL-3 of control group; 4: GAL-3 after stimulation with 40% SPSC.
Figure 3
Figure 3 GAL-3 protein of SW1990 cells detected by Western blotting. 1: GAL-3 of control group; 2: GAL-3 after stimulation with 40% SPSC.
Figure 4
Figure 4 Proliferation of PSCs detected by MTT assay. (A) Absorbance at 490 nm after exposure to different concentrations of GAL-3 MA; (B) Absorbance at 490 nm after exposure to different concentrations of recombinant human GAL-3 protein; (C) Absorbance at 490 nm after exposure to different concentrations of SSW; (D) Absorbance at 490 nm after exposure to 40% SSW plus different concentrations of GAL-3 MA (aP < 0.05).
Figure 5
Figure 5 Proliferation of SW1990 cells detected by MTT assay. (A) Absorbance at 490 nm after exposure to different concentrations of GAL-3 MA; (B) Absorbance at 490 nm after exposure to different concentrations of SPSC; (C) Absorbance at 490 nm after exposure to 40% SPSC plus different concentrations of GAL-3 MA (aP < 0.05).
Figure 6
Figure 6 Number of infiltrating cells in each group. aP < 0.05 vs control; cP < 0.05 vs 40% SPSC.
Figure 7
Figure 7 Infiltrating SW1990 cells. (A) Control group. (B) GAL-3 MA group. (C) SPSC group. (D) SPSC plus GAL-3 MA group. Compared with the control group, GAL-3 antibody had no significant effect on invasion of SW1990 cells. SPSC stimulated the invasion process of SW1990 cells, and this stimulation was partly inhibited by GAL-3 antibody.