Survey of molecular profiling during human colon cancer development and progression by immunohistochemical staining on tissue microarray

Figure 1 HE staining of 4-μm thick section of the tissue microarray.

Figure 2 Immunophenotype of the investigated antigens (p53, p21, cyclin D1, bcl-2 and bax) in colon cancer (original magnification x 200). Positive stainings of p53, p21 and cyclin D1 were located in the cell nuclei, while those of bcl-2 and bax were in the cytoplasm.

Figure 3 Immunophenotype of the investigated antigens (Cox-2 and nm23-h1) in colon cancer (original magnification x 200). The protein expressions of Cox-2 and nm23-h1 were detected in the cytoplasm.

Figure 4 Immunophenotype of the investigated antigens (PTEN, p-Akt1, β-catenin and c-myc) in colon cancer (original magnification x 200). Expressions of PTEN, p-Akt1, β-catenin and c-myc were detected in the cell cytoplasm.