Two distinct pathways of p16 gene inactivation in gallbladder cancer

Figure 1 Immunohistochemical staining of p16. A: Tumor cells showing diffuse strong positive staining of p16; B: Tumor cells showing diffuse moderate staining; C: Tumor cells showing partly weak staining.

Figure 2 Representative example of the results of microsatellite analysis showing LOH at D9S1748 and LOH at D9S942 (right, case 25; left, case 45). The scales on the top and left side of each figure represent the size (bp) and the intensity, respectively. N: Normal; T: Tumor.

Figure 3 Methylation status of the p16^INK4a gene by methylation-specific PCR. PCR products amplified by unmethylated (U) and methylated (M) specific primers. P: Positive control; N: Negative control. Methyaltion band is at 150 bp and unmethylated band is at 234 bp.