Viral Hepatitis
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 21, 2006; 12(7): 1043-1048
Published online Feb 21, 2006. doi: 10.3748/wjg.v12.i7.1043
Figure 1
Figure 1 pGBKT7- HBcAg cut by EcoR I and Pst I on 0. 9% agarose/EtBr gel. Lanes 1 and 3: Marker; lane 2: HBcAg fragment cut by EcoR I and Pst I.
Figure 2
Figure 2 Western blotting shows the expression of HBcAg in yeast. Lane 1 is negative control and lane 2 is HBV core protein.
Figure 3
Figure 3 Screening colonies cut by BglII on 0. 9% agarose/EtBr gel
Figure 4
Figure 4 The nucleotide sequence of C1 gene and relevent amino acid sequences.
Figure 5
Figure 5 C1 DNA fragment amplified by RT-PCR. Lane 1 is Marker and lane 2 is a 366 bp fragment of C1, amplified by RT-PCR.
Figure 6
Figure 6 Positive clones interactive with the HBcAg protein grew on media lacking leucine, tryptophan, histidine and adenine.