Identification of differently expressed genes in human colorectal adenocarcinoma

Figure 1 Digestion plasmids of EcoRI/Hind/II. Lane M: DNA size markers; 1-15 lanes: Fragment of about 500 bp after EcoRI/HindIII digestion.

Figure 2 Reduction of G3PDH after PCR-select subtraction. M: DNA size markers; 1-4 lanes: Subtracted PCR products as template, G3PDH3’ and 5’ as primers at cycles 18, 23, 28, 33; 5-8 lanes: PCR products not subtracted as template, G3PDH3’ and 5’ as primers at cycles 18, 23, 28, 33.

Figure 3 Increased c-myc after PCR-select subtraction. M: DNA size markers; 1-4 lanes (left): PCR products not subtracted as template, c-myc 3’ and 5’ as primers at cycles 18, 23, 28, 33; 5-8 lanes: No PCR products subtracted as template, c-myc 3’ and 5’ as primers at cycles 18, 23, 28, 33; 1-4 lanes (right): PCR products subtracted as template, c-myc 3’ and 5’ as primers at cycles 18, 23, 28, 33.

Figure 4 cDNA microarray hybridized with fluorescent labeled probes prepared from HCRAC (A) and total RNA from normal colorectal tissue (B).

Figure 5 Results of QRT-PCR. Blue/brown/:β-actin N/β-actinT; green/bright green: CaMT/CaMN; green/red: Hsp10T/Hsp10N; blue: Sox-9N/Sox-9T.