Viral Hepatitis
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Dec 28, 2006; 12(48): 7774-7778
Published online Dec 28, 2006. doi: 10.3748/wjg.v12.i48.7774
Figure 1
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Figure 1 Hybrid HBcAg-T protein showing HCV T epitope (hatched box) inserted between HBcAg aa 79 and 80, a region located at the tip of core particle surface spikes.
Figure 2
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Figure 2 The construction of plasmids pTrc-core-HCV (T) and pcDNA3. 1-core-GFP.
Figure 3
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Figure 3 Hybrid HBcAg-GFP protein showing the GFP protein (hatched box) inserted between HBcAg aa 79 and 80.
Figure 4
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Figure 4 Plasmid pTrc-coreNheI identified by restriction endonuclease NheI cleavage. Land 1: pTrc-coreNheI digest; M: λ-HindIII digest.
Figure 5
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Figure 5 The sequencing of plasmid pTrc-coreNheI PCR product.
Figure 6
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Figure 6 pcDNA3. 1-HBc-GFP expression in Hela cell line (confocal microscopy, 300 ×).
Figure 7
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Figure 7 PCR products of pTrc-core-HCV(T). Lanes 1-2: pTrc-coreNheI or pTrc-core-HCV (T) PCR product; M: pBR322 DNA-Msp I digest.
Figure 8
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Figure 8 The results of HBcAg-T and HBcAg by SDS-PAGE. 1: Marker; 2: Negative; 3: HBcAg; 4: HBcAg-T.
Figure 9
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Figure 9 Tumor regression trial in mice. 1: HBcAg immunized team; 2,3: Vacuity team; 4: HBcAg-T immunized team.
Figure 10
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Figure 10 The titer of HBcAb. 1: Vacuity team; 2: HBcAg immunized team; 3:HBcAg-T immunized team.
Figure 11
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Figure 11 Nonspecific lymphocytes prolifera-tive response. 1: Blank team; 2: HBcAg immunized team; 3: HBcAg-T immunized team.