Construction of an oral recombinant DNA vaccine from H pylori neutrophil activating protein and its immunogenicity

Figure 1 Electrophoresis of HP-NAP PCR products. Lane 1: 100 bp DNA ladder marker; lanes 2 and 3: PCR products of HP-NAP; lane 4: Blank control.

Figure 2 Identification map of recombinant plasmid pIRES-NAP. Lane 1: 100 bp DNA ladder marker; lane 2: PCR products templated on pIRES-NAP; lane 3: pIRES-NAP digested by endonucleases Xho I and Mlu I; lane 4: Blank control.

Figure 3 Western blot analysis of HP-NAP fusion protein expression. Lane 1: Protein standard; lane 2: COS-7 cells transfected with pIRES-NAP; lane 3: COS-7 cells without transfection as control.