Inhibition of hepatitis B virus production by Boehmeria nivea root extract in HepG2 2.2.15 cells

Figure 1 Effects of BNE on secreted HBsAg, HBeAg and HBV DNA from HepG2 2. 2.15 cell cultures. A: HBsAg level; B: HBeAg level; C: Viral DNA. The dotted line presents the limitation of this kit (3000 copies/mL). Data are expressed as mean ± SE of three independent experiments. ^aP < 0.05; ^bP < 0.01 vs the corresponding controls (Student’s t-test).

Figure 2 Cytotoxic effects of BNE on HepG2 2. 2.15 cells. Data are expressed as mean ± SE of three independent experiments (MTT assay).

Figure 3 Determination of the effective concentration of BNE anti-HBV activity. Cells were treated for 24 h. Data are expressed as mean ± SE of three independent experiments. ^aP < 0.05, ^bP < 0.01 vs the corresponding controls (Student’s t-test).

Figure 4 Effects of BNE on intracellular HBV DNA replication and transcription in HepG2 2. 2.15 cells. Cells were treated for 1 d. A: Northern analysis; B: Southern analysis.