Adiponectin and its receptors in rodent models of fatty liver disease and liver cirrhosis

Figure 1 AdipoR1 mRNA and protein and AdipoR2 mRNA in fatty liver disease. A: Expression of AdipoR1 in HepG2 incubated with 1 μmol/L or 10 μmol/L insulin for 4 h; B: Analysis of AdipoR1 in HepG2 treated with recombinant leptin for 24 h; C: AdipoR1 abundance in the liver of rats kept on a standard chow (SC) or a high fat diet (HF); D: AdipoR1 and AdipoR2 mRNA expression in the liver of rats kept on a standard chow (SC) or a high fat diet (HF). For normalization GAPDH mRNA levels were determined.

Figure 2 Hepatic adiponectin protein and mRNA in fatty liver disease. A: Adiponectin in the liver of standard chow (SC) and high fat diet (HF) animals. Concentration of adiponectin is ng per μg liver tissue; B: Adiponectin mRNA in the liver of rats kept on a standard chow (SC) or a high fat diet (HF). Fat tissue was analysed as a positive control; C: Primary hepatocytes were incubated with 5, 10 or 20 mg/L adiponectin for 24 h and adiponectin was determined in the cell lysates; D: Total systemic and hepatic adiponectin was calculated.

Figure 3 AdipoR1 and adiponectin in liver cirrhosis. A: AdipoR1 abundance in the liver of sham-operated or BDL mice; B: AdipoR1 and AdipoR2 mRNA expression in the liver of sham-operated or BDL mice. For normalization β-actin levels were determined; C: Systemic adiponectin in control and cholestatic animals; D: Adiponectin in the liver of sham-operated or BDL mice. Concentration of adiponectin is mg per g liver tissue.