Association of rare SPINK1 gene mutation with another base substitution in chronic pancreatitis patients

Figure 1 Detection of 5’UTR-215 G/A heterozygote in SPINK1 gene by BglI restriction endonuclease cleavage. 1: untreated prF-1R PCR fragment; 2: normal (-215 G/G) prF-1R PCR fragment treated with BglI endonuclease; 3: heterozygote (-215 G/A) prF-1R PCR fragment treated with BglI endonuclease [M: marker (Boehringer-Mannheim marker VIII); bp: base pairs].

Figure 2 Linkage between 5’UTR-215 G > A and IVS 3 + 2 T > C mutations in SPINK1 gene. A: sequencing of -215 G/A heterozygote; B: sequencing of IVS 3 + 2 T (normal allele) enriched by allele-specific PCR; C: sequencing of IVS 3 + 2 C (mutant allele) enriched by allele-specific PCR.