Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting

Figure 1 Putative PSC isolated from adult pancreas. A: Morphology of cultivated putative PSCs; B: Cells aggregate when saturated. bar = 100 μm.

Figure 2 Nestin and pdx-1 expressed in putative PSCs after 5 passages of cultivation. cDNA oriented from 4 individual donors (lane No. 1 to 4), 100 bp marker (M) and the plasmid cloned human nestin and pdx-1 gene with positive control (C) were shown.

Figure 3 The differentiation of putative PSCs (4 wk). A: Growth in Matrigel™; B: Dithizone stain; C-E: Immunohistochemistry staining by anti-insulin (C), glucagon (D) and somatostatin (E) immunoglobulins. bar = 100 μm.

Figure 4 Measurement of Insulin content and secretion in differentiated putative PSCs.

Figure 5 Cell doubling time of putative PSC.