Expression of macrophage inflammatory protein-1α in Kupffer cells following liver ischemia or reperfusion injury in rats

Figure 1 Lysozyme immunohistochemical staining of the Kupffer cells. The cytoplasms of the Kupffer cells were stained brown. The percentage of Kupffer cells in the adherent cells was over 90%.

Figure 2 Comparison of TNF-α production in supernatant of Kupffer cells. (n = 8, mean ± SE, ^aP < 0.05 compared with the next group, ^bP < 0.01 compared with the next group.)

Figure 3 Comparison of IL-1β production in supernatant of Kupffer cells. (n = 8, mean ± SE, ^bP < 0.01 compared with the next group.)

Figure 4 Immunohistochemical staining of MIP-1α in Kupffer cells in IRI group at 12 h’s interval SP × 400.

Figure 5 The synthesis of MIP-1α protein in Kupffer cells tested by immunohistochemical (n = 8, mean ± SE, ^bP < 0. 01 compared with control group).

Figure 6 A: Photograph of the MIP-1α mRNA expression in Kupffer cells tested by RT-PCR; B: Photograph of the GAPDH mRNA expression in Kupffer cells tested by RT-PCR.

Figure 7 Quantitative data of MIP-1α mRNA levels (n = 8, mean ± SE, ^bP < 0. 01 compared with control group).