Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hepatoma cells

Figure 1 Stably transfected cells showing green fluorescence under a fluorescence microscope (A) and cells without stable expression of the transfected gene (B).

Figure 2 P-gp activity in HepG2 cells (A1), HepG2/ADM cells (A2), HepG2/ADM/vec control clone cells (A3); HepG2/ADM/pGenSil-l/MDR-B clone 1 cells (A4); HepG2/ADM/pGenSil-l /MDR-B clone 2 cells (A5) Rhodamine123 efflux was measured by counting cells in the M1 region of the plot. The marker bar M1 was set to indicate the cells with high Rhodamine123 efflux; the marker bar M2 was set to indicate the cells with low rhodamine123 efflux. Data were presented as mean ± SD. ^aP < 0.05 vs untreated multidrug-resistant cell line HepG2/ADM.

Figure 3 shRNA-mediated suppression of MDR1 mRNA. He, sh1, sh2, con and He/ADM represent HepG2, HepG2/ADM/pGenSil-l/MDR-B clone 1, HepG2/ADM/pGenSil-l/MDR-B clone 2, HepG2/ADM/vec control clone and HepG2/ADM, respectively. The relative expression values were the mean of triplicate real-time PCR reactions. Bars indicate SE.