Inactivation of RASSF1A, the tumor suppressor gene at 3p21.3 in extrahepatic cholangiocarcinoma

Figure 1 Methylation specific PCR (MSP) analysis of RASSF1A in extrahepatic cholangiocarcinoma with methylated DNA-specific primers (m) and unmethylated DNA-specific primers (u).

Figure 2 Restriction enzyme digestion of the promoter CpG island of bisulfite-treated in 4 tumors using Restriction enzyme TaqI.

Figure 3 Frequency of methylation each CpG in RASSF1A promote in extrahepatic cholangiocarcinoma.

Figure 4 RASSF1A mutation analysis using SSCP in normal bile duct and 6 extrahepatic cholangiocarcinoma samples. It displayed that tumor 2 has a extra band, tumor 3 has a extra band with shifting. We consider that there are mutation in those two tumor samples.

Figure 5 LOH analysis of microsatellite loci D3S1568 and D3S4604 at the region of chromosome 3p21. 3 using the primers labeled with FAM and HEX.