Application of restriction display PCR technique in the preparation of cDNA microarray probes

Figure 1 Arrangement of all spots on gene chip. Positive controls, negative controls, empty controls, HCV probes.

Figure 2 RD-PCR patterns of HCV genes (A) and 1. 5% agarose gel electrophoresis of RD-PCR products from the white clones of HCV genomic fragments (B). M: MDL2 000 standard DNA ladder; lanes 1-10: PCR products of positive clones.

Figure 3 Scanning plots of hybridizing signals on gene chip of HCV (A) and modified gene chip (B). The samples were serially diluted (1.02×10⁵, 1.01×10⁸, 1.04×10¹¹ copies/mL) and used in microarray analysis.

Figure 4 Fluorescence intensity from cDNA microarray analysis as a function of serial dilution from 10⁴ to 10¹¹ copies of plasmid pCV-J4L6S (r = 0. 9819, ^bP<0.01)/mL (A) and comparison of the HCV RNA levels in 15 serum samples determined by cDNA microarray analysis and RT-PCR (Taqman) assays (B).