Induction of pancreatic duct cells of neonatal rats into insulin-producing cells with fetal bovine serum: A natural protocol and its use for patch clamp experiments

Figure 1 Morphological change of neonatal pancreatic duct cells cultured for 3-4 d (A), 5-7 d (B), 8-10 d (C and D) and more than 10 d (E), as well as identification of pancreatic islet cells by dithizone (F).

Figure 2 Insulin release curve (A) and MTT value curve (B) during the culture period of isolated adult islet cells and neonatal endocrine cells.

Figure 3 Identification of pancreatic β cells. A: Currents recorded from α or δ cells; B: currents recorded from β cells.

Figure 4 ATP sensitive potassium currents recorded in the absence (A), presence of diazoxide (B) and repaglinide (C).

Figure 5 Voltage-dependent potassium currents recorded on neonatal pancreatic β cells.

Figure 6 Voltage-dependent calcium currents recorded in the absence (A), presence of Bay K8644 (B) and nifedipine (C).