Nuclear factor-κB decoy oligodeoxynucleotides attenuates ischemia/reperfusion injury in rat liver graft

Figure 1 NF-κB activation in the liver graft. Lanes 1: Hepatic nuclear protein extracts from sham control rat; Lanes 2-8: Liver graft nuclear protein extracts on 0, 0.5, 1, 2, 4, 8 and 16 h after reperfusion.

Figure 2 Hepatic NF-κB activation (A) and NF-κB DNA binding activities presented as IOD value (B). Lanes 1 and 2: Hepatic RNA extracts from sham control group. Lanes 3 and 4: Hepatic RNA extracts from I/R group. Lanes 5 and 6: Hepatic RNA extracts from I/R + NF-κB decoy ODNs group. ^{b, d}P<0.001 vs sham group, ^{f, h}P<0.001 vs I/R group.

Figure 3 Hepatic expression (A) and the expression level (B) of cytokine mRNA after 4 and 8 h of reperfusion. Lanes 1 and 2: Hepatic RNA extracts from sham control group. Lanes 3 and 4: Hepatic RNA extracts from I/R group. Lanes 5 and 6: Hepatic RNA extracts from I/R + NF-κB decoy ODNs group. ^{b, d}P<0.001 vs sham group; ^{f, h}P<0.001 vs I/R group.

Figure 4 Serum levels of TNF-α (A) and IFN-γ (B) after hepatic ischemia/reperfusion. ^bP<0.001 vs I/R group.

Figure 5 Hepatic MPO content (A) and serum level of ALT (B). ^bP<0.001 vs I/R group.