Inhibitory effects of extracellular adenosine triphosphate on growth of esophageal carcinoma cells

Figure 1 Effects of various concentrations of ATP or ADO on survival fraction of TE-13 cells. ^aP<0.05, ^bP<0.01 vs 48 h groups.

Figure 2 Effects of ATP (A) and ADO (B) on cell cycle of TE-13 cells (n = 3). ^aP<0.05, ^bP<0.01 vs 0 mmol/L.

Figure 3 Fluorescence micrographs of TE-13 cells incubated for 48 h without treatment (A) and treated with ATP (B) or ADO(C) (×400).

Figure 4 Agarose gel electrophoresis of DNA extracted from apoptotic TE-13 cells treated with ATP (A) or ADO (B) for 72 h. Lane 1: control; lanes 2-5: 1, 0.3, 0.1, and 0.03 mmol/L, respectively; lane 6: marker.

Figure 5 ATP- or ADO-induced apoptosis of TE-13 cells detected by flow cytometry in control (A) and treated with ADO (B) and ATP (C) for 48 h, respectively.