Persistence of high CD40 and CD40L expression after restorative proctocolectomy for ulcerative colitis

Figure 1 Mean percentages with standard error of CD40L+ and CD40+ leukocytes in the mucosa of UC ileum, ileal pouch, and controls. ^aP < 0.05 vs controls.

Figure 2 Comparison of CD40L and CD40 leukocytes’ expression between pouchitis and healthy pouch mucosa. ^aP < 0.05 vs healthy pouch.

Figure 3 CD40 expression in B cells (CD20+) in controls (A) is homogeneously distributed under the cut-off. This expression is instead above the cut-off value, in several B cells in pouch patients with spondyloarthropathy (B), as in those with UC.

Figure 4 Flow cytometric analysis of CD40+/CD20+ cells in blood. ^aP < 0.05 vs controls.

Figure 5 Blood B cells in pouch patients with extraintestinal manifestations, such as spondyloarthropathy or pyoderma gangrenosum, present a higher expression of CD40 with respect to the other pouch patients. Instead no difference is present during pouchitis. Hfi, high fluorescence intensity. ^aP < 0.05 vs absence.