Hepatocytes targeting of cationic liposomes modified with soybean sterylglucoside and polyethylene glycol

Figure 1 Transfection efficiencies. A: Transfection efficiencies of FS entrapped in different types of liposomes incubated with HepG2 2.2.15 cells at 37 °C. --□--: free FS solution as control; -·-: N-liposomes; -▲-: C-liposomes; ---·---: C/SG-liposomes. Data represents the average of two wells; B: Transfection efficiencies of FS entrapped in different types of liposomes incubated with HepG2 2.2.15 cells at 37 °C for 24 h. Data represents mean±SD, n = 3.

Figure 2 Percent of inhibition on HBsAg and HBeAg secretion of free ODN and C/SG/PEG-liposomes entrapped ODN in HepG2 2. 2.15 cells culture medium incubated at 37 °C. The ODN concentration was 1.25 (A), 2.5 (B) and 5.0 mmol/L(C). __: free ODN; ---: C/SG/PEG-liposomes encapsulated ODN; o: HBsAg; ▲: HBeAg. Experimental values are given as the mean of two independent experiments.

Figure 3 Distribution in liver tissue and uptake amounts in hepatocytes and non-parenchymal cells of liver of ³H-labeled liposomes, after intravenous administration by tail vein in mice, at different time periods. A: 0.5; B: 4.0 h. The open bar represents C-liposomes and the filled bar represents C/PEG/SG-liposomes. Data represent mean±SD, n = 3. The statistical significance of differences was analyzed by Student’s t-test. ^bP<0.01 vs C/PEG/SG-liposomes.