Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

doi:10.3748/wjg.v11.i28.4351

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Jul 28, 2005 (publication date) through Apr 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. Jul 28, 2005; 11(28): 4351-4356
Published online Jul 28, 2005. doi: 10.3748/wjg.v11.i28.4351

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Figure 1 (PDF) RT-PCR analysis of HBV X gene mRNA expression in HL-7702. M: 100-bp DNA ladder; lane 1: HL-7702T; lane 2: HL-7702/pcDNA3T; lane 3: HL-7702/HBxT; lane 4: HL-7702/HBxS; lane 5: HL-7702/pcDNA3S; lane 6: HL-7702S.

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Figure 2 (PDF) Relative expression levels of apoptosis-associated gene mRNA in HL-7702 of different transiently transfected groups assessed by RT-PCR. A: Relative Fas mRNA expression levels; B: relative FasL mRNA expression levels; C: relative Bax mRNA expression levels; D: relative Bcl-2 mRNA expression levels; E: relative c-myc mRNA expression levels. ^aP<0.05 vs HL-7702/HBxT, ^bP<0.01 vs HL-7702/HBxT, ^dP<0.01 vs HL-7702, ^fP<0.01 vs HL-7702/pcDNA3T, P>0.05 between random two groups.

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Figure 3 (PDF) RT-PCR results of apoptosis-associated gene mRNA expression in HL-7702 of different transiently transfected groups. A: RT-PCR results of Fas/FasL mRNA expression; B: RT-PCR results of Bax/Bcl-2 mRNA expression; C: RT-PCR results of c-myc mRNA expression; M: 100-bp DNA ladder; lane 1: HL-7702; lane 2: HL-7702/pcDNA3T; lane 3: HL-7702/HBxT; a: Fas; b: FasL; c: Bax; d: Bcl-2; e: c-myc.

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Figure 4 (PDF) Relative expression levels of apoptosis-associated gene mRNA in HL-7702 of different stably transfected groups assessed by RT-PCR. A: Relative Fas mRNA expression levels; B: relative FasL mRNA expression levels; C: relative Bax mRNA expression levels; D: relative Bcl-2 mRNA expression levels; E: relative c-myc mRNA expression levels. ^aP<0.05 vs HL-7702/HBxS; ^bP<0.01 vs HL-7702/HBxS, ^dP<0.01 vs HL-7702, P>0.05 between random two groups.

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Figure 5 (PDF) RT-PCR results of apoptosis-associated gene mRNA expression in HL-7702 of different stably transfected groups. A: RT-PCR results of Fas/FasL mRNA expression; B: RT-PCR results of Bax/Bcl-2 mRNA expression; C: RT-PCR results of c-myc mRNA expression; M: 100-bp DNA ladder; lane 1: HL-7702; lane 2: HL-7702/pcDNA3S; lane 3: HL-7702/HBxS; a: Fas; b: FasL; c: Bax; d: Bcl-2; e: c-myc.

Citation: Zhang SJ, Chen HY, Chen ZX, Wang XZ. Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes. World J Gastroenterol 2005; 11(28): 4351-4356
URL: https://www.wjgnet.com/1007-9327/full/v11/i28/4351.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i28.4351